FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20167728
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC228700.089.93597120
GAGCACA373750.056.26703120
AGAGCAC522850.040.111183118-119
AAGAGCA800800.026.270437120
GAAGAGC958000.022.093388116-117
GGAAGAG1079950.019.38105118-119
CGGAAGA1235400.017.059153114-115
TCGGAAG1253100.016.64622116-117
ATCGGAA1442250.014.633337112-113
GATCGGA1471000.014.208736114-115
AGATCGG1608850.013.117483110-111
TAGATCG353950.011.75407110-111
GAGATCG636550.010.892973110-111
CACACGT57200.010.579968120
CACGTCT66400.010.481179120
AAGATCG524200.010.186052112-113
CTAGATC99450.010.145389116-117
CAGATCG510250.09.842439112-113
TTAGATC139800.09.648621108-109
TGAGATC233900.09.013805108-109
Produced by FastQC (version 0.11.5)