FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12702060
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC164750.072.17863120
GAGCACA261150.047.43661120
AGAGCAC347000.035.136738118-119
AAGAGCA536250.023.831774120
GAAGAGC576550.021.323175118-119
GGAAGAG691350.017.9949118-119
CGGAAGA808400.015.207665116-117
TCGGAAG824500.015.110081116-117
ATCGGAA964800.012.945418114-115
GATCGGA971400.012.929474114-115
AGATCGG1074200.011.611354112-113
CACACGT43150.011.509665120
CTAGATC62850.011.352199120
TAGATCG226500.010.840079118-119
CACGTCT51200.010.3831415120
ACACGTC49000.010.064369118-119
AAGATCG489100.09.789698116-117
TTAGATC99100.09.5289545120
ACGTCTG63000.09.326611120
GAGATCG343800.09.054325110-111
Produced by FastQC (version 0.11.5)