FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14172787
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG78550.010.540214120
TGGTCGC247950.08.396183120
AGATCTC454750.08.364367120
TAGATCT467800.08.220805120
GGTGGTC302200.08.080095120
TCTCGGT387950.07.8715186120
AGTGTAG634450.07.8486905120
GGTCGCC251350.07.8052244120
CTCGGTG371350.07.6902423120
GTAGATC627500.07.6201077120
AGAGTGT725550.07.4833794120
ATCTCGG419000.07.4743423120
TCGGTGG357500.07.43437120
GATCTCG444200.07.428491120
GTGTAGA624800.07.4129796120
CGGTGGT354800.07.389488120
GTGGTCG274850.07.3561516118-119
GAGTGTA676300.07.079132118-119
TGTAGGG1195100.07.0682993120
AAGAGTG792050.06.9005275120
Produced by FastQC (version 0.11.5)