FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13728381
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC132800.062.24193120
GAGCACA201900.044.372948120
AGAGCAC272800.034.422638120
AAGAGCA429450.021.805422120
GAAGAGC468650.019.953936118-119
GGAAGAG583050.015.915832116-117
TCGGAAG679850.014.206969120
CGGAAGA704350.013.434847116-117
ATCGGAA810350.012.096592120
GATCGGA805700.012.065149118-119
AGATCGG881600.011.022531120
TAGATCG211150.010.716125116-117
CTAGATC54350.09.625939116-117
TTAGATC90700.09.1929655116-117
AAGATCG389900.08.595711118-119
GAGATCG290950.08.226506116-117
CAGATCG302000.07.449107116-117
CCTAGAT19150.07.17108120
CACACGT44650.06.8836308118-119
CACGTCT49100.06.7923927120
Produced by FastQC (version 0.11.5)