FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15219549
Sequences flagged as poor quality0
Sequence length56-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1061550.012.388334120
CGTATCA498400.010.905937120
GTATCAT510200.09.912883120
GCCGTAT615100.09.773156120
CCGTATC582100.09.708813120
CGCCGTA648900.09.347301120
TGGTCGC838300.09.08902120
TCATTAA396100.09.0878105120
GGTCGCC792600.08.99996120
GGTGGTC1016650.08.662982120
ATCATTA462550.08.508597120
GTCGCCG784250.08.506738120
GTGGTCG913800.08.489072120
TCGCCGT735850.08.397707120
CATTAAA395800.08.048808120
TCGGTGG1179050.07.932804120
GCGTCGG82650.07.8395967120
CTCGGTG1256300.07.5787296120
CGGTGGT1119850.07.4815903118-119
TATCATT563450.07.400179120
Produced by FastQC (version 0.11.5)