FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15219549
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC206800.074.37524120
GAGCACA308400.049.255997120
AGAGCAC412350.037.779465120
AAGAGCA614550.025.24129118-119
GAAGAGC681000.022.70856118-119
GGAAGAG847450.018.296137116-117
CGGAAGA987400.015.891664116-117
TCGGAAG986250.015.72177114-115
ATCGGAA1158550.013.582342114-115
GATCGGA1179350.013.175011116-117
ACACGTC59850.012.59271120
AGATCGG1289700.012.181394112-113
CACACGT55600.011.976026120
CACGTCT57300.011.876118120
TAGATCG216050.011.366596114-115
TTAGATC95550.011.333842120
CTAGATC60250.010.917576110-111
AAGATCG677650.010.294752114-115
GAGATCG431150.09.06914110-111
TAAGATC293200.08.9240055110-111
Produced by FastQC (version 0.11.5)