FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11894963
Sequences flagged as poor quality0
Sequence length54-126
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT39350.027.902234
TCCGATC41250.027.6351763
CGATCTA12750.020.7049925
CTTCCGA81800.014.8947191
AAAAAAA3693450.013.680985120
TTCCGAT120800.010.3306822
CGATCTC40750.010.1590725
CGTATCA1274200.09.648032120
CGGTGGG7902.7808592E-59.11357120
CCGTATC1381950.08.973927120
ATCATTA1184600.08.934314120
CATGTCG28950.08.9147561
GTATCAT1210750.08.875146120
GCCGTAT1498950.08.70976120
TCATTAA1101150.08.55436120
CGCCGTA1608550.08.489305120
CATTAAA1059000.08.413271120
TATCATT1297150.08.219239120
CATTCCA84250.08.1212811
CATAGAC29600.08.1106911
Produced by FastQC (version 0.11.5)