FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11894963
Sequences flagged as poor quality0
Sequence length50-126
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC189150.0149.3762120
GAGCACA323650.084.569305120
AGAGCAC439800.063.20154118-119
AAGAGCA658200.041.151257118-119
GAAGAGC715550.038.588142116-117
GGAAGAG839750.031.728714116-117
CACACGT51600.030.998508120
CACGTCT53650.027.937626120
CGGAAGA994000.027.758997114-115
TCCGATC40650.027.7338373
ACACGTC56600.027.074383120
TCGGAAG1062500.025.116526114-115
CCGATCT42250.024.9583324
ACGTCTG61500.024.553482120
GCACACG62900.022.940004120
ATCGGAA1240100.022.680979112-113
CGTCTGA70000.021.73178120
GATCGGA1258650.021.497812112-113
CGATCTA12450.020.6865985
AGATCGG1350900.020.553026110-111
Produced by FastQC (version 0.11.5)