FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13629141
Sequences flagged as poor quality0
Sequence length51-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA5388750.016.158098120
TCTCGGG19950.011.127812120
TGATAGG38000.09.9473631
CATTAAA1558100.09.646359120
TGTTCGG62750.09.4661291
TGTCACG32450.09.4298871
TCATTAA1665800.09.41529120
TGTATAC70750.09.3286161
TGTACGG41000.09.0731651
ATCATTA1812500.09.040545120
TGTACTG67800.09.0265441
CGTATCA2140300.08.833336120
GTATCAT2043400.08.805906120
TGTAAGG48550.08.7744551
TGTACAG79500.08.679241
TATCATT2011600.08.667722120
GTTCGGG34850.08.6083162
ATTAAAA1646800.08.591202120
TGTTGCG57650.08.5342541
TGTAGGC29600.08.5135091
Produced by FastQC (version 0.11.5)