FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17132717
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG212250.09.666793120
TAGGGAA449700.09.485292120
TAGATCT155400.09.033767120
TGTAGGG502400.08.920208120
CGTGTAG596600.08.909523120
GTGTAGG556900.08.898296120
GAGTGTA229000.08.88113120
AGAGTGT261800.08.868402120
GTCGTGT683350.08.814436120
GTAGGGA467350.08.761217118-119
TCGTGTA642750.08.633833120
CGTCGTG774850.07.943899120
AGCGTCG877800.07.86654120
GTAGATC241250.07.8582125120
GAGCGTC963000.07.7997746120
AAGAGTG318150.07.6936464120
GCGTCGT824450.07.52056118-119
AGAGCGT1034700.07.2940745120
GTGTAGA228500.07.2727323120
AAGAGCG1142250.07.1535225120
Produced by FastQC (version 0.11.5)