FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17498744
Sequences flagged as poor quality0
Sequence length50-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC287500.0150.72676120
GAGCACA500250.088.13035120
AGAGCAC685900.063.868523118-119
AAGAGCA1021100.041.874683118-119
GAAGAGC1134550.038.46467116-117
GGAAGAG1349050.031.499256116-117
CGGAAGA1585050.027.600029114-115
TCGGAAG1672450.025.478893114-115
ATCGGAA1942850.022.63713112-113
CACGTCT66950.021.537773120
GATCGGA1992250.021.395233112-113
CACACGT61600.020.788006120
AGATCGG2141850.020.461752110-111
ACACGTC65200.019.145071118-119
TAGATCG577250.018.083338110-111
GAGATCG628400.017.185099110-111
CTAGATC123350.017.143291108-109
CAGATCG686350.016.314255110-111
AAGATCG643400.016.27431110-111
TTAGATC211350.015.759019108-109
Produced by FastQC (version 0.11.5)