FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15081203
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC198750.098.59134120
GAGCACA341500.057.4019120
AGAGCAC467750.042.33865118-119
AAGAGCA698850.028.270964120
GAAGAGC783950.025.222359116-117
GGAAGAG937900.020.983002120
CGGAAGA1081050.018.404886114-115
CACGTCT52550.017.336586120
TCGGAAG1147050.017.234787118-119
CACACGT48250.016.161377120
ATCGGAA1317800.015.181046112-113
TCCGATC60200.014.8153543
GATCGGA1369850.014.513384116-117
AGATCGG1513450.013.233555110-111
ACACGTC54750.012.973798118-119
ACGTCTG63150.012.348163120
TAGATCG351150.012.213942116-117
CCGATCT73150.011.5194854
AAGATCG582800.011.068566114-115
CTAGATC89500.010.999217110-111
Produced by FastQC (version 0.11.5)