FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14813248
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC226600.0100.77849120
GAGCACA349150.063.93887120
AGAGCAC464050.048.731552118-119
AAGAGCA676650.032.819214118-119
GAAGAGC764650.029.496544116-117
GGAAGAG937200.023.700127116-117
CACGTCT79300.021.220415120
CGGAAGA1102100.020.523043114-115
ACACGTC84000.020.141956120
TCGGAAG1110250.019.857834114-115
CACACGT78250.019.28452120
ATCGGAA1306300.017.524496112-113
GATCGGA1327900.016.881504112-113
AGATCGG1443450.015.9248295110-111
ACGTCTG99000.014.780665120
GCACACG98250.014.559857118-119
TAGATCG294050.013.878383110-111
CGTCTGA116150.013.779328120
CTAGATC65550.013.73337108-109
TTAGATC100550.013.272057112-113
Produced by FastQC (version 0.11.5)