FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15355617
Sequences flagged as poor quality0
Sequence length52-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG74050.010.535683120
TAGATCT455850.09.623651120
CTCGGTG357100.09.041424120
TGGTCGC208850.08.965293120
AGTGTAG626250.08.931257120
ATCTCGG389950.08.895355120
AGATCTC447900.08.883352120
GTGGTCG237200.08.855188120
TCGGTGG336950.08.851876120
GATCTCG426000.08.77653120
GAGTGTA678400.08.687004120
TCTCGGT360000.08.618515120
GTGTAGA614950.08.412245120
GTCGCCG219200.08.405087120
GGTCGCC203300.08.324462120
AGAGTGT740200.08.245486120
CGGTGGT331650.08.21524120
GGTGGTC282900.08.124754120
CGCCGTA181450.08.003921120
AAGAGTG822250.07.8679075120
Produced by FastQC (version 0.11.5)