FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15355617
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC193650.059.165638120
GAGCACA297650.038.86838120
AGAGCAC377400.031.473562120
AAGAGCA569000.021.637903120
GAAGAGC613850.019.644398118-119
GGAAGAG766600.017.243797120
CGGAAGA879600.015.013584120
TCGGAAG886950.014.853201118-119
GATCGGA1076750.012.405048120
ATCGGAA1068700.012.301663120
AGATCGG1179600.011.285031118-119
TAGATCG250600.010.733742114-115
ACACGTC60050.010.070811120
AAGATCG514150.09.435309120
CTAGATC75300.09.409901116-117
GAGATCG377850.08.698418120
CACGTCT61450.08.664685120
CAGATCG405250.08.434572114-115
CACACGT62400.08.358686118-119
TTAGATC111200.07.994505112-113
Produced by FastQC (version 0.11.5)