FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18227069
Sequences flagged as poor quality0
Sequence length51-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGGA76300.014.3907981
CTAGGGG5402.9039074E-713.33354951
AAAAAAA1940300.012.9463415120
CTTGGGT42050.011.2724781
CTTGGGG51700.010.9092681
CTTGGTA87950.010.8472481
CTAGGGC6653.588886E-610.8272421
CTTGGCG175600.010.4215811
CTTGGCA157800.010.2663261
CTTGGGC67000.010.0300131
CTTGGCC107400.09.83256151
CTTGCGA95500.09.2357511
CTTCCGA141900.09.2179091
CGTATCA798600.09.210309120
CTTGGTG134900.09.0290561
CTTGGAT106700.08.9411011
CTTGGAC68850.08.5404431
CTAGGTG9201.7919256E-58.4783981
GTATCAT788700.08.367464120
TCATTAA649500.08.313356120
Produced by FastQC (version 0.11.5)