FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15709855
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC230450.0120.246506120
GAGCACA390650.068.65507120
AGAGCAC541800.050.39981118-119
AAGAGCA781300.034.599007118-119
GAAGAGC907100.030.07899116-117
GGAAGAG1099350.024.58155116-117
CGGAAGA1298100.021.1193114-115
TCCGATC79900.020.989513
TCGGAAG1332700.020.304321114-115
CCGATCT87000.018.6797754
ATCGGAA1526050.018.049896112-113
GATCGGA1587350.017.111956112-113
CGATCTA27050.017.0832025
AGATCGG1732000.015.8351145110-111
ACACGTC66950.015.319786120
CACGTCT70050.014.898698120
TAGATCG456350.014.874557110-111
CTTCCGA115900.014.6242691
CACACGT60350.013.9393835118-119
CTAGATC99100.013.707813108-109
Produced by FastQC (version 0.11.5)