FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14258789
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC179300.084.340805120
GAGCACA292500.052.408745120
AGAGCAC401650.038.085205118-119
AAGAGCA593200.026.757763120
GAAGAGC680700.022.950027118-119
GGAAGAG857500.018.118166118-119
CGGAAGA1013250.015.569263116-117
TCGGAAG1007350.015.366693114-115
ATCGGAA1162450.013.546937114-115
GATCGGA1182450.013.111989112-113
AGATCGG1297600.012.108138112-113
TAGATCG291900.011.054167114-115
CTAGATC68850.010.587485108-109
TTAGATC102200.010.559136120
AAGATCG546550.010.133595112-113
GAGATCG416750.09.457159110-111
TAAGATC223450.08.699431120
CAGATCG400100.08.573604110-111
CACACGT42950.08.319729120
CACGTCT48250.08.146438120
Produced by FastQC (version 0.11.5)