FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17487596
Sequences flagged as poor quality0
Sequence length51-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA3099800.014.356807120
GCGTCGG150800.09.00808120
TCTCGGG46050.09.006262120
TCATTAA950150.08.932388120
ATCATTA1050350.08.5323515120
CGTATCA1233900.08.325057120
GTATCAT1194350.08.298779120
CATTAAA913450.08.166051120
CCGTATC1365500.08.002529120
GCCGTAT1471500.07.9734206120
TCCGATC99350.07.97146653
CGCCGTA1569600.07.8082433120
ATTAAAA1006300.07.532042120
GGTCGCC1867000.07.507742120
CGATCTA24950.07.45458275
TATCATT1198650.07.421547120
TTAAAAA999600.07.396121120
GTGGTCG2091650.07.376697120
GTCGCCG1848300.07.164191120
GGTGGTC2216200.07.1465554120
Produced by FastQC (version 0.11.5)