FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15460527
Sequences flagged as poor quality0
Sequence length68-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG101150.010.085215120
AGATCTC576200.09.154143120
TGGTCGC284350.08.905561120
TAGATCT594450.08.751971120
GTGTAGA785350.08.733435120
GTGGTCG312150.08.727595120
CTCGGTG453200.08.61972120
TCTCGGT476800.08.608391120
CGGTGGT411000.08.541145120
TCGGTGG421650.08.439266120
AGTGTAG813850.08.375988120
GATCTCG566150.08.3627205120
AGAGTGT951650.08.304445120
ATCTCGG521000.08.177541120
GTAGATC781900.08.165683120
GAGTGTA876950.08.108595120
GGTCGCC287300.08.103977120
GGTGGTC350500.08.0808325120
TGTAGAT806750.08.077797120
AAGAGTG1043850.07.6859126120
Produced by FastQC (version 0.11.5)