FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11708602
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC123500.063.122444120
GAGCACA187250.040.83818120
AGAGCAC246550.032.326714120
AAGAGCA399750.022.136984120
GAAGAGC432900.019.20246120
GGAAGAG546950.016.084736120
TCGGAAG621700.014.228771118-119
CGGAAGA639350.014.114003120
ACACGTC42850.012.822467120
ATCGGAA745950.012.755619120
GATCGGA750850.012.319411120
AGATCGG836800.011.30124118-119
CACACGT40600.010.906063118-119
TAGATCG210300.010.880966120
CACGTCT41650.09.932725120
AAGATCG301350.09.287957120
GAGATCG296050.08.930211118-119
CAGATCG296700.08.714569120
CTAGATC48600.08.578525108-109
TTAGATC86100.08.033117120
Produced by FastQC (version 0.11.5)