FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11719623
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC116900.068.63195120
GAGCACA178400.045.08454120
AGAGCAC237850.033.606888118-119
AAGAGCA381550.022.058863120
GAAGAGC413950.019.777302118-119
GGAAGAG487800.016.824156118-119
TCGGAAG570300.015.097259120
CGGAAGA566200.014.853215120
ATCGGAA663000.012.851114118-119
CACACGT31150.012.631915120
GATCGGA674950.012.618116120
AGATCGG752000.011.290541116-117
TAGATCG166250.010.630641120
CACGTCT33350.09.598882120
CTAGATC44650.09.335994110-111
AAGATCG336850.09.107444120
GAGATCG258300.08.46921118-119
TTAGATC81050.08.105441118-119
CAGATCG246800.08.012789116-117
TAAGATC132750.07.888121114-115
Produced by FastQC (version 0.11.5)