FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11850104
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC134900.070.97234120
GAGCACA214600.046.801918120
AGAGCAC283550.034.72928118-119
AAGAGCA427250.024.511143120
GAAGAGC492250.020.356863118-119
GGAAGAG609350.016.628742118-119
TCGGAAG699800.014.546706120
CGGAAGA710450.014.386889116-117
GATCGGA843200.012.799106120
ATCGGAA822000.012.764963120
AGATCGG936800.011.428902118-119
CTAGATC51500.011.231008120
TAGATCG208250.010.946287120
CACACGT43900.09.145227120
TTAGATC70700.08.88559116-117
GAGATCG392900.08.7117605118-119
AAGATCG329850.08.540649120
CCTAGAT18000.08.300564118-119
CAGATCG349750.07.62148118-119
CGCTAGA7901.1244701E-67.310934106-107
Produced by FastQC (version 0.11.5)