FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10484009
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC112350.071.04842120
GAGCACA171700.046.16606120
AGAGCAC227750.035.01797120
AAGAGCA351900.023.334883120
GAAGAGC389500.020.6007120
GGAAGAG484800.016.608418120
TCGGAAG557900.014.331172116-117
CGGAAGA568850.014.085808116-117
GATCGGA638100.012.589993114-115
ATCGGAA657350.012.513031120
AGATCGG705550.011.568622112-113
CTAGATC44750.010.401338120
TAGATCG158950.010.392554110-111
CACGTCT40050.010.234272120
ACACGTC40100.09.875019120
GAGATCG262800.09.454961112-113
AAGATCG254200.09.2560625112-113
CAGATCG231050.08.990269120
TTAGATC61750.08.823377112-113
GCACACG50500.08.804324120
Produced by FastQC (version 0.11.5)