FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14619968
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC190200.080.62127120
GAGCACA306350.051.214752120
AGAGCAC415200.037.301315120
AAGAGCA626350.025.03866118-119
GAAGAGC688800.023.466324120
GGAAGAG851550.019.046873120
CGGAAGA998500.016.317333118-119
TCGGAAG1022950.015.746758118-119
ATCGGAA1195950.013.69367116-117
GATCGGA1218800.013.282527116-117
AGATCGG1342700.012.168828114-115
TAGATCG294750.011.563585120
CTAGATC74850.010.43079112-113
AAGATCG587100.010.115169118-119
ACACGTC44050.09.652091120
GAGATCG409800.09.627523114-115
TTAGATC128300.09.399171114-115
CACGTCT44800.09.023758120
CAGATCG385200.08.99417112-113
CACACGT41100.08.988174120
Produced by FastQC (version 0.11.5)