FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12812119
Sequences flagged as poor quality0
Sequence length51-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG115250.010.254798120
AGAGTGT149200.09.529746120
TAGGGAA240450.09.331439120
TCGTGTA358750.09.113945120
CGTGTAG333100.09.02329120
GAGTGTA126000.08.927555118-119
TGTAGGG297750.08.68415120
GTGTAGG321150.08.425011120
GTCGTGT377600.08.293548118-119
GTAGGGA255400.08.22146120
GTGTAGA137650.08.193764120
TAGATCT88600.08.193198120
CGTCGTG435600.08.056933120
AAGAGTG171100.07.8541613120
AGCGTCG501600.07.582869120
AAAGAGT213350.07.4235754120
GGGAAAG294250.07.197141120
AGAGCGT589150.07.0975537120
GAAGAGC703250.07.0550094120
GCGTCGT469350.07.017418118-119
Produced by FastQC (version 0.11.5)