FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12812119
Sequences flagged as poor quality0
Sequence length50-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC118000.032.403744120
GAGCACA138000.027.039362120
AGAGCAC176000.024.798904120
AAGAGCA266100.017.187586120
GAAGAGC292950.016.85035120
TCGGAAG367650.014.028596120
GGAAGAG354050.013.508335120
CGGAAGA409100.011.878435118-119
GATCGGA449300.011.711814118-119
ATCGGAA456000.011.580176120
TAGATCG128100.011.373288120
AGATCGG534100.010.92271120
CTAGATC34950.010.553363120
CCTAGAT15500.08.725248118-119
GCTAGAT19850.08.671303120
TTAGATC53000.08.11912120
ACACGTC34350.07.158468120
CACACGT29500.06.876679118-119
CGCTAGA8600.0046529966.4332566120
GAGATCG274350.06.397171118-119
Produced by FastQC (version 0.11.5)