FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12012539
Sequences flagged as poor quality0
Sequence length50-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA674300.014.03524120
TCATTAA252350.010.844333120
CGTATCA375300.010.633699120
GCGTCGG109100.010.396288120
CATTAAA251300.09.743364120
GGTCGCC646450.09.71042120
ATCATTA297650.09.637476120
CCGTATC445000.09.615477120
TGGTCGC673050.09.585227120
TATCATT355350.09.508092120
GCCGTAT481250.09.377528120
GTATCAT364900.09.292142120
GTGGTCG739550.09.283244120
CGCCGTA533350.09.26038120
GGTGGTC802200.09.111836120
TCGGTGG946150.08.721355120
CGGTGGT898600.08.67529120
TCTCGGT1080100.08.662105120
TCGCCGT627700.08.566377120
ATCTCGG1174450.08.395456120
Produced by FastQC (version 0.11.5)