FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12324925
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC187650.0124.12379120
GAGCACA320600.070.33305120
AGAGCAC430900.053.23773118-119
AAGAGCA650450.035.208313120
GAAGAGC726300.031.611027116-117
CCGATCT97900.030.2897384
TCCGATC109300.027.3169783
CGATCTA25200.026.2843975
GGAAGAG874500.026.194094116-117
CTTCCGA120900.025.0212291
CGGAAGA1024000.022.639345114-115
TCGGAAG1067950.021.409067114-115
ATCGGAA1248800.018.57766112-113
GATCGGA1284200.017.857986112-113
TTCCGAT182100.016.9278052
AGATCGG1362950.016.864159110-111
TAGATCG334100.015.517751110-111
CACACGT37400.014.773422120
CTAGATC53100.014.432105112-113
GAGATCG549100.013.898229110-111
Produced by FastQC (version 0.11.5)