FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16487673
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC225200.089.88555120
GAGCACA357350.057.263977120
AGAGCAC484250.042.166992118-119
AAGAGCA719300.029.096447120
GAAGAGC845900.024.20031116-117
GGAAGAG1013850.020.412956118-119
CGGAAGA1173900.017.590157114-115
TCGGAAG1175000.017.469194116-117
ATCGGAA1371500.015.180071112-113
GATCGGA1426300.014.430327114-115
AGATCGG1564000.013.288963110-111
TAGATCG271550.012.589184110-111
CACGTCT63250.011.608143120
CACACGT61200.011.287204118-119
CTAGATC56350.010.998545112-113
ACACGTC67950.010.92141120
GAGATCG676650.010.442836112-113
AAGATCG567450.09.933828114-115
ACGTCTG83850.09.886135120
CAGATCG569950.09.502696112-113
Produced by FastQC (version 0.11.5)