FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13651145
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1736400.016.117922120
CCGATCT88800.014.1938854
TCCGATC109500.012.7713483
CGATCTA21200.012.1731245
CTTCCGA126800.011.786321
CATTAAA598800.010.690298120
TCATTAA622400.010.593398120
ATCATTA715000.010.555572120
CGTATCA872850.010.337497120
GTATCAT832550.010.29023120
CCGTATC994250.010.257949120
GCCGTAT1069750.09.836815120
ATTAAAA649450.09.62563120
TATCATT824900.09.607463120
CGCCGTA1146350.09.37315120
GCGTCGG82750.09.207525120
GGTCGCC1415300.09.101033120
GTCGCCG1343000.09.059393120
TGGTCGC1512600.08.78927120
TCGCCGT1262800.08.708334120
Produced by FastQC (version 0.11.5)