FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14033359
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC167500.072.82586120
GAGCACA262050.045.700382120
AGAGCAC344050.035.556183120
AAGAGCA531950.023.663483120
GAAGAGC594850.020.740187118-119
GGAAGAG729900.017.264965120
CGGAAGA851650.015.180605120
TCGGAAG841750.015.110242118-119
ATCGGAA992750.013.121056120
GATCGGA1010800.012.665768116-117
AGATCGG1115500.011.652271120
TAGATCG290200.010.834561118-119
CACGTCT52550.010.0580015120
CTAGATC66000.010.010379120
CACACGT46700.09.977662120
ACACGTC53350.08.994676120
AAGATCG371950.08.835725114-115
TTAGATC101350.08.593761108-109
GAGATCG401800.08.549852110-111
CAGATCG413800.08.520793114-115
Produced by FastQC (version 0.11.5)