FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16093828
Sequences flagged as poor quality0
Sequence length52-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG76650.012.605552120
GGTCGCC636450.010.664646120
GTCGCCG614550.010.507591120
CGCCGTA525100.010.18319120
GGTGGTC824500.09.673473120
GTGGTCG753150.09.625729120
TGGTCGC685100.09.606962118-119
TCGCCGT589000.09.577688120
AAAAAAA1028250.09.554283120
CGTATCA416300.09.456829120
CCGTATC483200.09.377091120
GCCGTAT501650.09.331295120
GTATCAT447600.08.90279120
CTCGGTG1041500.08.551116120
TCGGTGG983750.08.522359120
CGATCTA21250.08.4701885
CGGTGGT922050.08.35716120
ATCTCGG1201050.08.344562120
TCTCGGT1112200.08.088479120
ATCATTA408100.07.9851184120