FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13244454
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC150450.070.74799120
GAGCACA240150.046.355843120
AGAGCAC321300.036.04445120
AAGAGCA497250.023.595488120
GAAGAGC552750.021.10695120
GGAAGAG677250.018.40581120
CGGAAGA811250.015.3411875120
TCGGAAG827100.015.3025055120
ATCGGAA965800.013.016011118-119
GATCGGA998700.012.633479118-119
AGATCGG1119400.012.261681120
TAGATCG267200.011.039339120
CTAGATC64950.010.261536118-119
GAGATCG459550.08.816693118-119
TTAGATC98550.08.771758120
AAGATCG359000.08.584093120
CAGATCG418900.08.577438118-119
CACGTCT44800.08.248633120
CCTAGAT25700.08.216467116-117
CCCTAGA8701.8135324E-87.5849175118-119
Produced by FastQC (version 0.11.5)