FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12020944
Sequences flagged as poor quality0
Sequence length59-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA425450.09.815575120
GAGTGTA474900.09.185172120
AGAGTGT517050.09.179076120
TAGATCT314200.09.1471195120
AGTGTAG438700.09.06778120
GTGGTCG174900.08.988071120
GATCTCG298000.08.919541120
AGATCTC297050.08.867271120
TCGGTGG227600.08.752274120
AAGAGTG574350.08.733427120
GTAGATC423000.08.595819120
CTCGGTG250400.08.338731120
ATCTCGG276700.08.261727120
TCTCGGT253300.08.219575120
GCGTCGG89550.08.107273120
TGTAGAT451600.08.064729120
GGTGGTC201300.07.958341120
CGGTGGT229050.07.7800283120
TGTAGGG959500.07.7353463120
AAAGAGT674950.07.6717463120
Produced by FastQC (version 0.11.5)