FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11467342
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC191100.0119.197754120
GAGCACA325850.068.9002120
AGAGCAC441050.051.416622118-119
AAGAGCA638350.034.57579118-119
GAAGAGC729900.030.971888116-117
GGAAGAG883450.025.033308118-119
CGGAAGA1032550.022.073374114-115
TCGGAAG1086150.020.259596116-117
ATCGGAA1266000.018.047546112-113
GATCGGA1315950.016.903008114-115
AGATCGG1430750.015.962156110-111
CACGTCT42550.014.980347120
TAGATCG309750.014.209048110-111
CTAGATC68700.013.841373112-113
CACACGT36100.013.720002118-119
GAGATCG615650.013.699801112-113
ACACGTC41700.013.256147118-119
CAGATCG470150.013.0024395110-111
TTAGATC122700.012.114404108-109
TGAGATC257600.011.484594114-115
Produced by FastQC (version 0.11.5)