FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15560739
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152550.061.74793120
GAGCACA231250.042.30878120
AGAGCAC309900.032.49913120
AAGAGCA479550.021.188505120
GAAGAGC550350.018.625265120
GGAAGAG693850.015.887685120
TCGGAAG798050.014.021458120
CGGAAGA824600.013.054614118-119
GATCGGA956450.012.247214120
ATCGGAA944150.011.797595120
AGATCGG1056650.011.357991120
TAGATCG259450.010.381981118-119
CTAGATC67450.09.948181118-119
TTAGATC106350.09.313894120
GAGATCG432700.08.72846120
AAGATCG435450.08.3945120
CACGTCT54800.08.279705120
CCTAGAT26250.07.303543110-111
CTCTAGA14204.4640637E-67.2006006120
ACACGTC56300.07.1510406120
Produced by FastQC (version 0.11.5)