FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15019680
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC230700.096.38236120
GAGCACA399200.057.99607120
AGAGCAC562600.041.45405120
AAGAGCA817400.028.727125118-119
GAAGAGC964650.023.851908118-119
GGAAGAG1138650.020.615602116-117
CGGAAGA1310300.017.666876116-117
TCGGAAG1390850.016.853333114-115
ATCGGAA1590400.014.65743114-115
GATCGGA1653400.014.247378112-113
AGATCGG1827350.012.781973112-113
TAGATCG465050.011.746599120
AAGATCG520100.011.088005110-111
GAGATCG652600.011.021895110-111
CTAGATC87800.010.854795112-113
CACGTCT71750.010.714669120
TTAGATC193950.010.709862120
CAGATCG530350.010.47514118-119
ACACGTC76150.010.289714120
AGAGATC234650.09.7973175120
Produced by FastQC (version 0.11.5)