FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14818320
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC233400.0114.852264120
GAGCACA404650.064.45691120
AGAGCAC557600.047.680634118-119
AAGAGCA824550.032.123703118-119
GAAGAGC936650.028.348019116-117
GGAAGAG1127050.023.413692116-117
CGGAAGA1260300.021.208223114-115
TCGGAAG1322000.019.9576114-115
ATCGGAA1523950.017.58211112-113
GATCGGA1566250.016.889881112-113
AGATCGG1705400.015.727059110-111
CACGTCT58200.014.810068120
TAGATCG381850.013.713374112-113
AAGATCG702700.013.5917015110-111
GAGATCG492000.013.490549110-111
CAGATCG428450.012.684955110-111
ACACGTC57850.012.66472118-119
TCCGATC58250.012.4936723
CTAGATC111550.012.402506110-111
CACACGT53350.012.359675118-119
Produced by FastQC (version 0.11.5)