FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15886746
Sequences flagged as poor quality0
Sequence length55-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG6951.6243575E-98.63233890-91
TGGTCGC274000.08.585568120
TAGATCT490400.08.198943120
GTGGTCG302000.08.127367120
AGATCTC491950.07.916939120
AGTCTAG8953.7471182E-107.708822788-89
TCGGTGG388650.07.5815215120
GTGTAGA626050.07.4864435120
ATCTCGG461250.07.4420605120
GAGTGTA672100.07.3127975120
GATCTCG509150.07.2841063120
CGGTGGT399300.07.0937557120
AGAGTGT729400.07.075649120
CTAGGAT11452.7284841E-117.07361792-93
GGTGGTC342800.07.0358224118-119
GTAGATC647600.07.0241847120
AGTGTAG631700.06.9919853120
CGTATCA191700.06.918369120
TCTCGGT425550.06.8536077120
CTCGGTG428250.06.822778118-119
Produced by FastQC (version 0.11.5)