FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15886746
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC161450.055.05083120
GAGCACA240500.037.735035120
AGAGCAC306050.029.284166118-119
AAGAGCA464900.020.173933120
GAAGAGC519150.019.135815120
GGAAGAG633850.015.365429118-119
TCGGAAG717150.014.03269120
CGGAAGA755100.013.156348120
GATCGGA861200.012.660531120
ATCGGAA863150.012.182926120
AGATCGG969000.011.08899118-119
TAGATCG222650.010.700564116-117
CTAGATC54600.010.096909114-115
TCTAGAT25800.08.872043114-115
TTAGATC76350.08.798483120
GAGATCG445550.08.060497120
CAGATCG412300.07.801884120
AAGATCG353450.07.693742120
CCTAGAT22150.07.568583114-115
CGCTAGA12052.5297006E-57.504547120
Produced by FastQC (version 0.11.5)