FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13743920
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC164600.074.32214120
GAGCACA249200.049.3178120
AGAGCAC328250.037.463863118-119
AAGAGCA503900.023.87154118-119
GAAGAGC554450.022.052105116-117
GGAAGAG710250.016.915472120
TCGGAAG813450.014.839120
CGGAAGA837300.014.788154114-115
ATCGGAA965850.012.922039112-113
GATCGGA967050.012.5222225112-113
AGATCGG1056400.011.860582110-111
TAGATCG185950.010.740268118-119
CTAGATC51300.010.4734335118-119
ACACGTC49500.010.428448120
CACACGT53500.09.913098120
CACGTCT52650.09.670215120
AAGATCG490400.09.560157120
CAGATCG359000.09.041079120
TTAGATC72050.08.833047120
GAGATCG347950.08.603691110-111
Produced by FastQC (version 0.11.5)