FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19559012
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC199400.064.65903120
GAGCACA299250.044.96438120
AGAGCAC398700.033.615856120
AAGAGCA642600.021.167458118-119
GAAGAGC709750.019.284595120
CCGATCT110850.017.8274864
CGATCTA28950.015.8142995
GGAAGAG909800.015.175177120
TCCGATC135000.014.8959143
TCGGAAG1032850.014.142638120
CGGAAGA1088500.012.829786120
GATCGGA1196650.012.269523118-119
ATCGGAA1225900.012.201655120
AGATCGG1297300.011.530108120
TAGATCG318950.011.122631120
CTTCCGA192800.010.79976751
CTAGATC69400.010.776664120
GATCTAG8801.5117257E-79.8747156
AAGATCG474700.08.816244116-117
TTAGATC101100.08.7886915118-119
Produced by FastQC (version 0.11.5)