FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13802969
Sequences flagged as poor quality0
Sequence length54-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG102600.010.818514120
TAGATCT634000.09.908335120
TGGTCGC296400.09.513994120
GGTGGTC358850.09.095554120
TCGGTGG429900.09.015883120
ATCTCGG547350.09.010522120
AGTGTAG859400.08.943284120
AGATCTC615250.08.923037120
GTGGTCG324750.08.831245120
AGAGTGT1007450.08.6593275120
GGTCGCC309000.08.601783120
GAGTGTA921600.08.593591120
TCTCGGT492000.08.548622120
GTGTAGA824850.08.401372120
CTCGGTG478750.08.371647120
TGTAGAT844750.08.253177120
CGGTGGT407850.08.252882120
AAGAGTG1103800.08.207857120
GATCTCG609400.08.014293120
GTAGATC808850.07.981555120
Produced by FastQC (version 0.11.5)