FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13604556
Sequences flagged as poor quality0
Sequence length58-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG57650.08.962033120
TGGTCGC229650.08.789825120
GGTGGTC278100.08.511433120
CTCGGTG331300.08.359627120
TCTCGGT356700.08.269625120
GATCTCG395950.08.117473120
GGTCGCC224700.08.101155120
GTGGTCG248150.08.013479120
TAGATCT436000.07.7989717120
AGATCTC425350.07.7823825120
GTGTAGA578700.07.651057120
GAGTGTA630000.07.6192746120
ATCTCGG384050.07.6024914120
AGAGTGT678800.07.469784120
TCGGTGG330550.07.3789787120
GTAGATC592300.07.3638053120
GTCGCCG234150.07.3123693120
AGTGTAG587200.07.2333717120
CGCCGTA213250.07.099356120
AAGAGTG748800.07.0442715120
Produced by FastQC (version 0.11.5)