FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13604556
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC134600.066.4213120
GAGCACA205550.045.27504120
AGAGCAC277450.034.24885120
AAGAGCA442450.021.625853118-119
GAAGAGC499350.020.063349120
GGAAGAG642700.015.700206120
TCGGAAG730100.013.8565445120
CGGAAGA752050.013.460477118-119
ATCGGAA857750.012.49538120
GATCGGA846350.012.249809118-119
AGATCGG913500.011.654104120
TAGATCG245650.011.12054120
TTAGATC97800.09.756192120
AAGATCG373100.09.704001120
CTAGATC55850.09.595266120
GAGATCG286200.08.982129116-117
ACACGTC46050.08.798901120
CAGATCG265200.08.575748120
CACGTCT47750.08.075046120
CCTAGAT19200.07.3060822110-111
Produced by FastQC (version 0.11.5)