FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16224303
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC155450.069.725075120
GAGCACA249200.044.829697120
AGAGCAC335700.033.200653120
AAGAGCA537200.021.108402118-119
GAAGAGC594250.019.38142120
GGAAGAG736200.016.16736120
CGGAAGA865000.014.333345120
TCGGAAG851950.014.246524120
GATCGGA1020250.012.203428120
ATCGGAA1029700.012.180154120
TAGATCG297000.011.4030485120
AGATCGG1103800.011.306894118-119
CTAGATC66200.010.350025120
TTAGATC120500.08.60403118-119
AAGATCG419850.08.594905120
GAGATCG375150.08.273629116-117
CAGATCG397400.07.8927927114-115
ACACGTC49150.07.7004223120
CACGTCT50300.07.524369120
ATAGATC163350.06.9258347118-119
Produced by FastQC (version 0.11.5)