FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12322831
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC139500.074.501945120
GAGCACA222150.046.753185120
AGAGCAC296350.035.942253120
AAGAGCA469400.023.256824120
GAAGAGC514850.020.741419118-119
GGAAGAG668600.017.233183120
TCGGAAG761300.014.732733120
CGGAAGA786500.014.0056118-119
GATCGGA943450.012.976934120
ATCGGAA919800.012.408435120
TAGATCG230150.011.762254120
AGATCGG1021000.011.711474120
CACACGT42800.010.965383120
CTAGATC52650.010.464128116-117
CACGTCT47000.010.130216120
ACACGTC44600.09.912814120
AAGATCG386400.08.889427118-119
GAGATCG374850.08.84579118-119
CAGATCG366100.08.509131118-119
TTAGATC87850.08.323121118-119
Produced by FastQC (version 0.11.5)