FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831387
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences21640293
Sequences flagged as poor quality0
Sequence length100
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1096530.5067075570557201Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC295620.1366062834731489Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA108400.053.745052
GATCGGG111500.053.1378331
ATCGGAA309200.044.264362
AGAGCGT269500.043.8182268
GATCGGA313750.043.682481
GAGCGTC273500.042.8848239
TCGGAAG323200.040.7742083
AAGAGCG311250.039.6658747
CGGAAGA350300.038.4787144
CCGTATC201600.036.88451848-49
CGGGAAA100500.036.5740174
CGCCGTA203400.036.45191646-47
CGGGAGA185200.034.4847074
TGGTCGC210800.034.35918842-43
GTCGCCG223150.033.05449744-45
GTATCAT226100.032.69851750-51
TCGTGTA228200.032.6239814-15
AGCGTCG241000.031.8932110-11
TCGGGAA79100.031.6770233
CGTCGTG239000.031.55330312-13
Produced by FastQC (version 0.11.5)