FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004831454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831454
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences30063414
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2061420.6856905872366991TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG483070.16068368017018958TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)